A special high speed, short throw drive system solved this problem. Providing a dequate mixing for oxygen transfer was an issue in the smaller wells. They needed special options and adaptation to accommodate large numbers per chamber and to stop wells drying out over time. The need for thousands of screening experiments to provide rapid screening for specific biological activity drove an increased use in 96-well plates. Then, things changed and applications such as algal biofuel and massively parallel screening in microtiter plates came to the fore. The excellent temperature distribution, a certified antimicrobial surface and options such as darkening for insect cells infected with Bacculovirus made the Mulitron the “ideal home” for these cultures.Ģ012-2017: Multiple, parallel experiments and green applications This led to a new generation of Multitron with a larger 50 mm shaking throw for gentle mixing plus the ability to measure and control parameters such as gassing with CO 2 and percentage relative humidity. Also, production of viral vectors for vaccines and recombinant work using both insect and mammalian cells were common. Microbial and fungi/yeast hosts for r ecombinant protein production were limited in their ability to provide complete glycosylation. 2008-2011: Cell culture applications come to the foreīy the early 2000’s, the need for growth of mammalian and insect cells in suspension culture was clear. However, applications were changing with the rise of cell culture for mammalian or insect cell lines. This system was a genuine revolution and met the changing needs of researchers engaged in microbial work. This provided its own design challenges to keep the top deck accessible, providing easy flask handling, prevent any spillages from moving down the stack, and allowing easy access for cleaning and servicing. The first Multitron provided a “game changer” in that it made use of vertical space in the laboratory by stacking up to three incubation chambers. The alternative, an incubator shaker was more flexible, but lacked capacity, both in number of flasks and their maximum size. These systems were simple, effective for their main task but extremely inflexible for accommodating several users with different needs e.g., different growth temperatures. The main application for these units was often large-scale screening for antibiotics. In previous decades, the need for high capacity and throughput was met by free-standing, large shakers housed in constant-temperature rooms. The Multitron development dates to the 1990’s and came from the need for maximum shake flask capacity for the minimum space in the laboratory. In addition to shake flasks, test tubes and microwell plates were being used with shakers to provide good mixing and oxygen transfer.ġ991: Focus on fungal and microbial applications By the mid 1960’s, the shaker and its temperature-controlled sibling, the incubator shaker, were in common use as a standard laboratory tool for the biosciences. This made the shake flask useful for seed train production when Stirred Tank Reactors (STR’s) first came into use in the 1940’s for antibiotic production. Key parameters like shaking throw, an orbital movement for good growth and speed range were established early. The top unit stays in a comfortable working height of 1.40 m, while the new door mechanism and fast, automatized start-stopįunction keep interruptions in the cell culture process at a minimum.To provide a little context from a historical perspective, the use of orbital shakers for submerged culture in shake flasks dates to the mid 1930’s and concentrated on fungal culture. By applyingĪ three layer configuration, more than 50 liters or 23,000 batches can be grown parallely – even if the base is only 1 meter in width. As was the case for its predecessor, the footprint for this shaking incubator is very small compared to any other shaking incubator on the market. The new Multitron also meets the requirements for increased efficiency in the biotech industry. The new UVĭecontamination function, its condensate free direct steam incubation and its antibacterial coating help Multitron meet the stringent requirements for cell culture applications. The hygienic design of the new Multitron has also been improved with rounded, interior corners of the chamber, which has fewer components making it much easier to clean. Which guarantees comparable cell culture conditions in all batches. The new temperature control concept offers the possibility for the users to obtain the best possible gradient free temperature uniformity, The new Multitron offers even better conditions for growth of microorganisms, cell culture and phototrophs than before.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |